LOCUS EY194165 372 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab03e08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY194165 VERSION EY194165.1 GI:159498364 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 372) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 841 PolyA tail found inside read...SR being truncated to 523 Potential full insert read...vec-to-vec length: 444 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..372 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcca gttctgatgc aaacaaagtt ggcagacctg caaaggagtg agaatagtgg 61 tggaattttc aatctcctgg agaccaaact aaagcaaata caatgggaaa aaatgggcaa 121 ggaattggcg gagaggacgg ccgatctggc aagggacagt gtccgaaagg ccgaaaagct 181 agcgcgtgac ccggaaacgc ggcaaaagtt ggcaactgcc atgaaagagg cggctgaaac 241 gaccagagaa gttgcccgaa aaaccatggg ggcggctgaa ggacgcatga aggagaagag 301 gaagtagtat ggacacaccc cattttagtt gcatatattg tctgttttct gtaataaaaa 361 atgaagtcgg ta //