LOCUS EY194164 590 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab03e06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY194164 VERSION EY194164.1 GI:159498363 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 590) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 814 was extended to value 815 (,) This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 817 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..590 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggaa aaagatgcaa tgacgcacga ggaggaagcc cacgcgccta aaaggggatc 61 gtcggaccag acaggccgca aatactccat cccgttcttt atcacacctc aaattgggtc 121 gcagcggatt caaattcgca agcatctgca aggagtactt ctgaacaccg ccttagagca 181 atctcccaaa aaaacggctc ctattggcca agacgaagcg agcattcact gtttgaatga 241 gtttgatggg tcttacgatc gcgtgaaaaa gcaccttttc tacatgctgg ggaaagatga 301 gtataacagg gaaggaatga caactgtgac tgggcttgga aaatatgcgg tgacttgctt 361 ccaccagatg aagcacccag aaaatccagc taaactaatc gcaatggaag gtatcaaggt 421 ctaccgagag acggatcgca cgtataaaaa cggggaaatt gtgcatggcc ctcccatcat 481 ctgcaaagtc gcgattgaat ttaaatatcc atttgatgtt gtgtatttgg agcaaacaga 541 cggaaagccg ttttgcgatg aattacccct tcttttggaa ccgaaaatag //