LOCUS EY194162 536 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab03e04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_492322.1| RAN (nuclear import/export) related RAN-4 (15.1 kD) (ran-4) [Caenorhabditis elegans] sp|Q21735|NTF2_CAEEL Probable nuclear transport factor 2 (NTF-2) pir|T23921 hypothetical protein R05D11.3 - Caenorhabditis elegans emb|CAA99890, mRNA sequence. ACCESSION EY194162 VERSION EY194162.1 GI:159498361 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 536) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 794 was extended to value 794 (,) This trace has been recalled with phred original value before phred recall for SL was 151 original value before phred recall for SR was 797 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..536 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggtac atatttttca aaataaatta aattaaatac acatggccgc ctacaatccg 61 aactatgaat ctattggaca agcatttgtt caacatttct acaacaaatt tgatgtgcca 121 gacgctcaag tacgcaccgc tggacttcat gacctctatg atgcggagca ttccttcatg 181 acatttgagg gaaaccaggt taagggacga caggcaattt tggagaaatt tggtgcattg 241 ccatttcgct caattgcaca cgggatcact aaggcggaca gtcagcctct tccagatgga 301 tcgattctag tgacagttat tggccaactc aagactgatg acgatcccat taactcgttc 361 aatcattgct tcatcctgag accgaattcg actggctcat tcttcatttc caacgaaatt 421 ttccgcctgg tcttgcattg atctcgtgga cggaggcatc aaccacgttg tgttatgctg 481 ttacgtttgg gcgatgatac ttcgatgatt tttgattaaa ttggggtttt atctaa //