LOCUS EY194160 285 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab03e02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to gb|AAN05584.1| ribosomal protein L30 [Argopecten irradians], mRNA sequence. ACCESSION EY194160 VERSION EY194160.1 GI:159498350 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 285) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 479 PolyA tail found inside read...SR being truncated to 436 Potential full insert read...vec-to-vec length: 357 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..285 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggac tggagtgcac gaataccacg ggaacaacat cgagctgggc actgcttgcg 61 gtaaattgtt ccgcgtccaa atgctctcca tcaccgaccc cggcgattct gacataattc 121 ggagcatgcc cacggaggct tgaaggattt gatttgttgt tcgatgtccg ttttgagctg 181 ttaaattttg ttggaataag agaaactcaa ttttcgctcc taaataatgt attagaatac 241 actcatttgt gagatttctc tgaaagtttg caattatttt aaaga //