LOCUS       EY194159                 312 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab03e01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_504575.1| CalReTiculin CRT-1 (45.6 kD) (crt-1)
            [Caenorhabditis elegans] sp|P27798|CRTC_CAEEL Calreticulin
            precursor pir|S25851 calreticulin precursor - Caenorhabditis
            elegans emb|CAA42159.1| calreticulin [Caenorhabditis elegans]
            gb|AAD147, mRNA sequence.
ACCESSION   EY194159
VERSION     EY194159.1  GI:159498349
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 151
            original value before phred recall for SR was 508
            PolyA tail found inside read...SR being truncated to 464
            Potential full insert read...vec-to-vec length: 384
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcca gaatacaagg gagaatggaa accaaagcgg atcaagaatc cggactatgt
       61 gggtccatgg aaacatccag aaattgaaaa cccggaatac gcgccagacg actttctgta
      121 tgctcgcgac gattggggcg caatcggaat cgacatttgg caggtgaaga gtggcactat
      181 cttcgacaac attttggtga ctgacagcga agaggatgca aagaagcatg ccgcggaaac
      241 attcgcttta cttcgaaagg cagaaacaaa gcaaaaagag gaagaggagg cgagacagaa
      301 ggccgaggat ga
//