LOCUS EY194158 501 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab03d12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY194158 VERSION EY194158.1 GI:159498348 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 501) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 689 PolyA tail found inside read...SR being truncated to 652 Potential full insert read...vec-to-vec length: 573 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..501 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcgg cgacctcaat tgcggagtac ctggtcaaga cgttcggaat tagtgcccgt 61 ctgggcctga gggacttctc caattcggag cacgaactgg gcattttggc aataaacgaa 121 cggggacccg caacgagtga ggcgattggc caggagatcc aaaaagtgct gcaagagtcc 181 tacgatcggg caaaggaaat tctcgtgaaa cgcaaaaggg aacaccagat gttggcggaa 241 gcgctactcg aacatgagac gttgaccaag acagaagtgg aaaaagtggt taccggcgaa 301 aaattgaaca agccccaaaa gaccattgcg cctccgagga aggcccgccg ccccgccacg 361 gaacctggtg tgaatttgcg ctacaacaag gagggcgatg aatgagtaga agaagaagga 421 ggacatcatg ttgatggtgt tcttcattaa taaatgagcg gattgcttga ataaaccctt 481 ttccgttgaa aaaaaaaaat a //