LOCUS       EY194157                 429 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab03d11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_502917.1| Glutamine synthetase (43.6 kD)
            [Caenorhabditis elegans] emb|CAB60321.1| Hypothetical protein
            Y105C5B.28 [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY194157
VERSION     EY194157.1  GI:159498347
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 619
            PolyA tail found inside read...SR being truncated to 580
            Potential full insert read...vec-to-vec length: 501
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggac aacgacggct acggttactt cgaggaccgt cgtccatcat ccaactgcga
       61 tccgtacttg gtgactgacg cactggtgcg cacttgctgt cttggtgtga agaaactgag
      121 ccgctcgtac atgcccgagg atgcggagca cttgcgcaag atgatcagtg agcttcgcgg
      181 cggcaagatt gacgagtagg ctgccgttgg attatggtcg gattgcagca gcggactcag
      241 atttgagagt gatgtagggg cgacgatcca ctcccacact cctttttagc attcacttaa
      301 tagcctttta actttactaa accaaacacg taaccaatta attctatgat tctacttcat
      361 catgtgtact atttttacta acgctcaaga acaaatgcag gcatttttaa ataaaatcaa
      421 tcttttcca
//