LOCUS EY194156 401 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab03d10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to gb|AAC24580.1| ADP/ATP translocase [Heterodera glycines], mRNA sequence. ACCESSION EY194156 VERSION EY194156.1 GI:159498346 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 401) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 796 was extended to value 796 (,) This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 806 PolyA tail found inside read...SR being truncated to 552 Potential full insert read...vec-to-vec length: 473 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..401 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcat catctacaag aacacgcttg attgcgcgat caagatcatc aagaatgagg 61 gcctcttcaa gggcatgttc aagggcgcgt tgtcgaacgt gttccgtggc actggtggcg 121 ccctggtgtt ggcgttctac gatgaaatcc agaagtacat ccactgagcg ggggacccgc 181 aaggaagatg ggcccttgac tgtttggctg cacccctcga cgacatataa gaacaatcag 241 gggattgaca tttgcgtgtc cgccgcacca ttatacctat tttcgataat tttgttgtgt 301 gatccttata gcaaccgccc cgcatcagac accgttatta ttattatgat gtttttggat 361 agacgaccca cactcgttgc tctggataaa aataactagg a //