LOCUS EY194155 340 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab03d09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY194155 VERSION EY194155.1 GI:159498345 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 340) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 815 was extended to value 816 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 533 PolyA tail found inside read...SR being truncated to 491 Potential full insert read...vec-to-vec length: 412 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..340 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggaa ccgaccaaat acgaacgagc gctctggaaa gatgcagtcg agacaatcaa 61 ggatattcac acaatcaaag agcgccgcta cggacaacac attgcgaata cgctgaagcc 121 gggtaaacaa attgcgaaga gggcaatgct gcagaaggcg agaaagaaag tgcatctgat 181 cagagcgcca gtggcagatg accaagaaga ggaagaggtg gagactgagg aggatgaagg 241 aaagccggaa ctggaggaag gtgaagcgca gatggaggtc aattaagcaa agtgattttg 301 tttcttgttt aacatttccg cgataaatcg taactggtca //