LOCUS       EY194134                 549 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab03b10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194134
VERSION     EY194134.1  GI:159497989
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 827 was extended to value 827 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 736
            PolyA tail found inside read...SR being truncated to 699
            Potential full insert read...vec-to-vec length: 621
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggacc tataaatgga ttcgaaatct ccaagtaatc tatcaaatgt gccatctttt
       61 aagagcaaga aaatggcaaa tactctgcgc aaacgggccc aatttagcga tgaagaagaa
      121 ccgaataaaa tggataatga tacgagcgat aatagcgatt cttcatctga atcggttggt
      181 ccacccgaaa tggtcagact caaattaata gagaaaagaa aacagctgag aggacgcaac
      241 cttattcata caacaagtaa aaagcgaaga aaggaaattg atacgctagc gaatggaagt
      301 tcgtcttctg aagatgatgg aggcaaaaat cccacatttg atcctgatgt taaatttggt
      361 tcgagcggta ttcaaagggt tggaccgatg gatgtgggtg caacagcgcg aagcgaagtt
      421 gacacggagt attccaaaga ttcgcaatca cagttcgaac ggattcaaga aatgttaaag
      481 aaagagagaa aggaacaaga attgcggttg gcgctagatg aggaggaccg gaagaagtac
      541 ggtgaacga
//