LOCUS       EY194132                 353 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab03b08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to gb|AAK72124.1| beta-actin [Chrysophrys auratus], mRNA
            sequence.
ACCESSION   EY194132
VERSION     EY194132.1  GI:159497987
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 818 was extended to value 819 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 550
            PolyA tail found inside read...SR being truncated to 505
            Potential full insert read...vec-to-vec length: 425
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggccg gcggcaccac catgtacccg ggaatcgctg accgcatgca gaaggagatc
       61 tccgcgctgg cgccttccac catgaagatc aagatcatcg cgccgcctga gcgcaagtac
      121 tccgtctgga tcggtggctc catcctggcc tcgctctcta ccttccagcg gatgtggatc
      181 tcaaagcagg agtacgacga gtcgggtccc tcgattgttc accgcaaatg cttctaagaa
      241 gggaaagaca acggcaaatg acaaagacgg acacgaaaca atatctctct tttttcgttt
      301 aaataagatg tggtcatgga tcaatcgttt aaaatctgaa taaatatgga cta
//