LOCUS       EY194126                 227 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab03b02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194126
VERSION     EY194126.1  GI:159497981
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 801 was extended to value 801 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 422
            PolyA tail found inside read...SR being truncated to 378
            Potential full insert read...vec-to-vec length: 299
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcat cctccccaaa aaaattctca tgtaattaag atgcgccacc tctgcatttt
       61 cctatctcgc gcaatccccg tgcaaagaat tctttttcta gtcttcacac ttccctgatt
      121 ctgagttcat tccagatccc ctttcccgtc atccattcca gtgaacttga ctgtcaatct
      181 tgcggtaatt ttggagggtt cgccgataaa cttggagagc gtgatga
//