LOCUS EY194119 395 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab03a07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_492767.1| Translationally controlled tumor protein (20.5 kD) [Caenorhabditis elegans] sp|Q93573|TCTP_CAEEL TRANSLATIONALLY CONTROLLED TUMOR PROTEIN HOMOLOG (TCTP) pir|T21352 hypothetical protein F25H2.11 - Caenorhabditis elegans emb|CAB020, mRNA sequence. ACCESSION EY194119 VERSION EY194119.1 GI:159497955 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 395) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 147 original value before phred recall for SR was 586 PolyA tail found inside read...SR being truncated to 545 Potential full insert read...vec-to-vec length: 467 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..395 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggaa gaaagttatc ggacatctgg aaaaggatgg caaacccgcc agtgatattg 61 atgccttcaa gaagaagatc caggcttggg ttgtttcgct actcaacaaa gaacgattca 121 aaaatctggc attcttcatc ggggaaaata tggccgatgg aaagggtgag ggtcaggttg 181 caattgtcga ataccgtcaa gtcgatggcg aagaagtgcc gaccctggtg ttggtgaagg 241 aagccttaac catcgagaaa tgttgatgtt gcagtggtgg gacggacgat tgatacacgt 301 caatgacaca acacattttt gccagttctt tcgaacttgc atcgtttgtt atcttccata 361 tatggttgac tgaagataaa aatgctcgct gttga //