LOCUS       EY194115                 322 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab03a02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to emb|CAA70693.1| cathepsin L-like cysteine proteinase
            [Heterodera glycines], mRNA sequence.
ACCESSION   EY194115
VERSION     EY194115.1  GI:159497930
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 517
            PolyA tail found inside read...SR being truncated to 474
            Potential full insert read...vec-to-vec length: 394
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggct cggaacaggg cgactactgg attgtgaaga acagttgggg aacgaagtgg
       61 gcgagaaggg ctacatccgc atggctcgca accgcaacaa caactgcggc gttgcctcgc
      121 acgcttccta cccgctggtc tgaacggtcc ctcgtttggt ttgggggatg tccattgatc
      181 cgattcagca gtccatcgat gcacataaga ttaagtaaac ttgcttttcg ttttagtaac
      241 cttaaaatcg tatcttgcaa gtcacatata tccacatgca acaaccattt tgtaaatttt
      301 caataaatgg cgaactttga ta
//