LOCUS       EY194107                 329 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab02h05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_497346.1| Putative protein, with a coiled coil-4
            domain, nematode specific [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY194107
VERSION     EY194107.1  GI:159497907
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 152
            original value before phred recall for SR was 525
            PolyA tail found inside read...SR being truncated to 483
            Potential full insert read...vec-to-vec length: 401
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggaca ctaaaaagac agcagacaaa aaggcgaata atgaatcgga gctgatcacg
       61 tccgccaaga tggatgatgc ggaagagttt gaggaattcc caatctacga tgcaggcggt
      121 gccggaatgg agagcgggga aaccacactc gccgaaatta acgtttggga ggacaactgg
      181 gatgacgaga caatcgaaac tgacttccac aagcagctgg ccgaggagtt gaagaagtgc
      241 ggtcagaaga cctaatccaa gtccatttaa aagtactctt tgctaagttt aactcgtgaa
      301 tttggatgga ggataaaatt aaagtgaca
//