LOCUS       EY194098                 446 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab02g07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to gb|AAP53847.1| putative speckle-type protein [Oryza
            sativa (japonica cultivar-group)], mRNA sequence.
ACCESSION   EY194098
VERSION     EY194098.1  GI:159497852
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 832 was extended to value 832 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 638
            PolyA tail found inside read...SR being truncated to 597
            Potential full insert read...vec-to-vec length: 518
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggaa ctgttggtgg ccgccgacaa gtaccagctg gaccaactga aggcactctg
       61 tgagctgcat atctttcggg ggctgaacaa ggacaacgtc tgcgcaatgt tggcactcgc
      121 gaagcgccag aatgccgaac agttgaagtc tggcgcaatc gagtacatca acatgcaggg
      181 agatgaggtg atggaaagcg acggatggaa cagaatggtc tcctcacatc cggagctgct
      241 caagcaactg ttccgggcat cccgcaaacg gggcggacag gattctggtg gcggagttgg
      301 tcgaccgcca aaagtgcgca tactatgatg cctttgagct gtcgattatg agattttaaa
      361 ttgcttgagg aaagagaacg tttggttgaa tatatgaagt ttgcggcatt tatttgaact
      421 actggcataa aaatttgtaa aaatga
//