LOCUS       EY194094                 200 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab02g03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194094
VERSION     EY194094.1  GI:159497831
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 200)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 341 (,)
            original QR value of 716 was extended to value 717 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 489
            PolyA tail found inside read...SR being truncated to 351
            Potential full insert read...vec-to-vec length: 272
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..200
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggg acagacaaca acggaagaag agaaaggggg cgtccgttgc agtgcaaacc
       61 agcatgcatc tggcggggca gtgataattt ttgggagaag gaatggattt caaaactcat
      121 ttttccattg gcgaatttga tcacaatatg tacaaatgca attcatattt gttgcaacat
      181 tgaaaaatgg gcctccttca
//