LOCUS       EY194086                 414 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab02f06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194086
VERSION     EY194086.1  GI:159497823
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 851 was extended to value 851 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 151
            original value before phred recall for SR was 610
            PolyA tail found inside read...SR being truncated to 565
            Potential full insert read...vec-to-vec length: 486
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggatc ctccgtgaga aggtgcaatg gccgcatgga atgccttgac gggtccgatg
       61 aacacgactg cacatgcgaa gaatgcaatc gccagccaac gccaatgtac atgtgcacgg
      121 aggcgcagcg ctgctttcca ctggacaagg tgtgctcgcc gtacagtttg tgcccgaatg
      181 ccacccagtt ggacaagctg ttctgcgctg cgcgtgggaa cgactacttc tgattgattg
      241 gcacaacgga agaacaccat ccatccttga ctttgccgaa gttgaaagtg tgtgatcact
      301 ccacccccaa aataattgac cctttcgaat tgacaccgcc aaccataata accaaccaaa
      361 catattgacc taccacacaa taattctcta gtagtacaaa cttacgcata agta
//