LOCUS       EY194082                 300 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab02f01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_723494.1| nicotinic Acetylcholine Receptor alpha
            30D CG4128-PA [Drosophila melanogaster] gb|AAF52817.2| CG4128-PA
            [Drosophila melanogaster], mRNA sequence.
ACCESSION   EY194082
VERSION     EY194082.1  GI:159496802
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 495
            PolyA tail found inside read...SR being truncated to 452
            Potential full insert read...vec-to-vec length: 372
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggtg cggacgagaa ctttgacgcg cgcttcgacg tcaacttctt ggtcagtcac
       61 gacggcagtg tgacccatac accgccggca atcatcaagt cctcgtgctc catcgacatc
      121 acttggtttc cgttcgacga acaaatgtgc ctgctcaagt tcggctcctg gacccacacg
      181 aagaactcgc tggacctgtg cattgacggt gcggcgctgg acactcaacg gcacagcatg
      241 ggtccacaat gaaaaatggt tcaattgcaa tttatgcgtc caataaatta ttgaactgta
//