LOCUS       EY194075                 587 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab02e04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_799506.1| hypothetical protein [Bacillus
            megaterium] gb|AAO52795.1| hypothetical protein [Bacillus
            megaterium], mRNA sequence.
ACCESSION   EY194075
VERSION     EY194075.1  GI:159496764
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 620 (,)
            original QR value of 822 was extended to value 822 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 770
            COMM   possible reversed clone; protein similarity on positive
            strand
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggat agtacaatga cataagcttg cctgactgcg aggctgacaa gccaagcaga
       61 gacgaaagtc ggtcatagtg atccggtgat tctgtatgga agggtcatcg ctcaacggat
      121 aaaaggtacg ccggggataa caggctgatg gtgttcgaga gttcatatcg acgacaccgt
      181 ttggcacctc gatgtcgact catcacatcc tggggctgga gaatgtccca agggttcggc
      241 tgttcgccga ttaaagtgat acgtgagttg ggtttagaac gtcgtgagac agttcggttt
      301 ctatctgccg tgggtgaagg aaatttgaga agatctgact ctagtacgag aggaccgaga
      361 tggatatacc tctggtgtac cagttgttat gccaatagca tcgctgggta gctatgtata
      421 gatgggataa ttgctgaaag catataagca agaaaccctc ttcaagaaga tttcccaatt
      481 aaggccgtgg gagaccacca cgttgatagg ctaggtgtgg aagcatggta acatgtgaag
      541 ctaactagta ctaatagcct gattgattta tttacttgat agaaaaa
//