LOCUS       EY194069                 497 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab02d09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194069
VERSION     EY194069.1  GI:159496758
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 825 was extended to value 826 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 151
            original value before phred recall for SR was 689
            PolyA tail found inside read...SR being truncated to 648
            Potential full insert read...vec-to-vec length: 569
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggaa acggaaacag ttcctccatt cctcccaatc gacctcttcc caaatgaagt
       61 gctctgctgc gctgctctgt gtgcttgtgc tggttctggc ggcctccgtc tcgctgccgg
      121 ccctggccca ggacgacccg tccccgggcc ttgcagggga aagggccgtc cgctcgccct
      181 gcaagcgctc tccgtccccc ctgtgcaagc gctctccgct gtgcaaacgc tcgccgcgct
      241 gtcgccgcga cccacaatcg tggggctgct gcacctactg ctgttgaaca cggtgctggc
      301 ctccgccctg ttggaggatg ggcggagaga actgccgagg gatggatgga cgatgggcac
      361 ctgagaacgc ataacaccca ataaatagta ataatgggtc cgacactgtg atagccatat
      421 tcagcatttt caacattagt caaaatgccc atttccaata aatcaaaaca ttcaactaaa
      481 taaagtgttg accacca
//