LOCUS       EY194068                 365 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab02d08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_502842.1| Putative secreted or extracellular
            protein precursor, nematode specific (32.5 kD) [Caenorhabditis
            elegans] emb|CAB70225.1| Hypothetical protein Y73F8A.6
            [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY194068
VERSION     EY194068.1  GI:159496757
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 839 was extended to value 840 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 151
            original value before phred recall for SR was 559
            PolyA tail found inside read...SR being truncated to 516
            Potential full insert read...vec-to-vec length: 437
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggca gcaggagcgg aactacgcgg cggtgctgga cgagctgaag aacaagttcg
       61 cgggactgcg ccagggctgt tacccgcgcc ccaaggggtg cctgtgcgta gtgggaaagg
      121 acgccactgg acgcgagatc accgaacgac gcatgaagga cagcgactgc aagtgtcgca
      181 aggacgagcg caatgcggag tgccccgcgg cgggagcgtg aaagaactat cgtattagat
      241 gagcaaatga acggtccatt ctgttcggaa agagattctt cacagatact ttcgcagttt
      301 tctgtttgct ccgtcccctc tgttccgttt gcgccgattc ccgtacagac agcacatttg
      361 gcgga
//