LOCUS EY194066 161 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab02d04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY194066 VERSION EY194066.1 GI:159496723 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 161) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 838 was extended to value 839 (,) This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 356 PolyA tail found inside read...SR being truncated to 312 Potential full insert read...vec-to-vec length: 233 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..161 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggga gacaggaatg cggaatgaca gagcacacgc ctagtcaaca aacaaacgaa 61 acatgggaca aacaccggtg caacagatca cttgtttaat ccacatttat cgatcgaatg 121 tccatatcac ggaatcacat ggataagctc aagcattcgg a //