LOCUS       EY194053                 543 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab02c03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_497700.1| Myosin light chain MLC-4 (19.9 kD)
            (mlc-4) [Caenorhabditis elegans] sp|Q09510|MLRH_CAEEL Probable
            myosin regulatory light chain pir|T20273 hypothetical protein
            C56G7.1 - Caenorhabditis elegans emb|CAA86772.1| C. elegans MLC-4
            pro, mRNA sequence.
ACCESSION   EY194053
VERSION     EY194053.1  GI:159496681
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 745
            Potential full insert read...vec-to-vec length: 542
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggat ggttttatca aagaacacga tcttaaggac atgtttgcat ctctcgggaa
       61 ggaagtatca aacaaattca tcgaggatat gatgaatgag gcatccggac ctatcaattt
      121 cgccatgttt ctgacactct ttggcggaaa actgactgga acagatccgg aagatgtgat
      181 aaagaatgcc tttcaatgtt ttgacgagga taacagtggg actatttctg aagatcgtct
      241 ccgagaactt ttaaccacaa tgggagatcg ctacaatgat gagcaggttg atgcgctgtt
      301 cagagatgcg ccaatcaagg atgggcgctt caactacatg gaatttacgc gaatgttaaa
      361 acacgggacc aaagaaaagg acgaggacaa caatgggaag taacttgatg ttggaagcga
      421 accgcaatcg attgactaat ggaatgttat ccacttgaaa aagcaatcat tcattccttt
      481 atcgacatat atatcgtata tcccgctttt acacaatgag accattgagt aaagtgctca
      541 ttc
//