LOCUS       EY194046                 307 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab02b07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194046
VERSION     EY194046.1  GI:159496647
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 821 was extended to value 822 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 153
            original value before phred recall for SR was 503
            PolyA tail found inside read...SR being truncated to 460
            Potential full insert read...vec-to-vec length: 379
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggc aaaggcactt tgcgattttc tcgtgaaaga gggcccaaac ggaaagtggg
       61 cacagcgact ggatggggag ctgaggccaa actatccgat ccaattccgc tcagtgcacg
      121 caatcagcgc aaaggaaaag cgattgggca atgtgaagac tacgctgagg gacatacacc
      181 gcaaactgaa cccaatcgaa tcggtcacat tcgaagaaga cctgttcgag aagaaacgaa
      241 ccgataaaat attagtatag tcattggatc tgaacattca ttcatttctt tgaagccttt
      301 aaacaga
//