LOCUS EY194045 358 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab02b06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY194045 VERSION EY194045.1 GI:159496646 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 358) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 553 PolyA tail found inside read...SR being truncated to 509 Potential full insert read...vec-to-vec length: 430 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..358 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggat ggtcgtggtc atcatcatcc tggctctgct ggccattttc tactacgagt 61 acaattatta cacgggcgaa agccacagca atacggatgg aaggagcgac cgatgcgagg 121 tggaggaatg gaccccgatg ggcgggagaa agacagactc agtggtgcag atgtacggca 181 ctgacgacac cggaggaacg gaaaattggg cattcgatgc gggagagaag cagaaatccc 241 caccgagatc ttagtatcat ttgatagctc ttggaattcc ctatcaaatg atatatcgaa 301 acttcgtgtt tctacaattt ttgaccatgt tatttgcttt tgaataaaaa taatgtca //