LOCUS       EY194044                 270 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab02b05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194044
VERSION     EY194044.1  GI:159496645
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 841 was extended to value 841 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 464
            PolyA tail found inside read...SR being truncated to 421
            Potential full insert read...vec-to-vec length: 342
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggtgg gaagaacaaa aaagtgagat acgcaaagca gccgccgaac atttgttcaa
       61 cgcgatcgac gttgacagcg atggaaaggt cacagcggcg gagatgaaaa agcacgtggg
      121 ttccgaagag gaggcagaag agagtccgct tctaaaagaa gtgttcggca acggcgatca
      181 tttggagtca gcagcctttt tggaggcatt cgacaaagtg cgggagtaca taagagagat
      241 gagagaggag aaaaagaagc aacaacgaga
//