LOCUS EY194043 393 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab02b04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to emb|CAC08539.1| CCHC zinc finger protein [Trypanosoma brucei], mRNA sequence. ACCESSION EY194043 VERSION EY194043.1 GI:159496644 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 393) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 155 original value before phred recall for SR was 593 PolyA tail found inside read...SR being truncated to 546 Potential full insert read...vec-to-vec length: 530 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..393 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggga cgcgatgatc gccgtgacga ccgcgatgac cgccgcggcg gaggcggtgg 61 tggccgtgat ggttgttaca actgcggcaa gagtggacac ttctctcgtg aatgtccgga 121 aggcggtcgc agtggtggac gcggaggcgg cggtcgctgc tacaactgca acgagtctgg 181 acatatgagt cgtgattgcc cgaaggaatc gcgctgaacg cgcacagcaa tgtaaccgat 241 gcgatgtgga gggcgaggct ggagcgaata agaagaattt ttcattttgc agtcgacgtt 301 ttcgattgaa tgtttattta attctataat ttacacgtgg cgaggccccc tcgatctctc 361 acattgttga accggataag attttgttac cca //