LOCUS EY194034 381 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab02a05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY194034 VERSION EY194034.1 GI:159496606 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 381) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 842 was extended to value 843 (,) This trace has been recalled with phred original value before phred recall for SL was 152 original value before phred recall for SR was 578 PolyA tail found inside read...SR being truncated to 533 Potential full insert read...vec-to-vec length: 453 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..381 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggac aatgccgccg actggacgct cgcacgcgca gctcttctct cggatgcgga 61 acatttgcgc gcgaatgaac agactgaagc gaaaacgcgc cccgcagcct ggcttaggtg 121 gctccccgga atcgctggtg gctttcctct gctcgaacac gaatctgttc tgacgcggaa 181 aggcaatcac agaatataca tatctataca tatgtgtgta cgagcacgtt gttttgtgac 241 cagccaagtc gtgatcgatt cgagattcct cctaacagcc gccattgaac caaccatctc 301 ccacatgccc gagtttatcc gcgagatatg gccattaaac attttagttg aatgaacgaa 361 tggatgataa acaaaaacac a //