LOCUS EY194032 271 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab02a03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_495781.1| Putative protein, nematode specific [Caenorhabditis elegans] pir|T21240 hypothetical protein F22B5.4 - Caenorhabditis elegans emb|CAA90356.1| Hypothetical protein F22B5.4 [Caenorhabditis elegans], mRNA sequence. ACCESSION EY194032 VERSION EY194032.1 GI:159496604 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 271) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 822 was extended to value 823 (,) This trace has been recalled with phred original value before phred recall for SL was 147 original value before phred recall for SR was 463 PolyA tail found inside read...SR being truncated to 422 Potential full insert read...vec-to-vec length: 343 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..271 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggca aacagtattt ttcgatccac ttggcgggac ccaccagcgt atagaaatca 61 tggagaagct acaggatgag atgttggagg cggccaagca gcgtggaacc cgcgatgccg 121 gtggctacat tgttactaac tagaacggga aggccagcaa agcagatgta aagatgaaac 181 gaatgaccag agactaattg accattcatt gatcattttc tggggcttca tttctggtcg 241 tagtgtaaaa ataaaaactg ttctagttat a //