LOCUS EY194031 292 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab02a02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY194031 VERSION EY194031.1 GI:159496603 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 292) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 152 original value before phred recall for SR was 489 PolyA tail found inside read...SR being truncated to 445 Potential full insert read...vec-to-vec length: 364 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..292 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggca attggatccg atgaggcgtc caacagcctc ccaagcgctg aacaacgggt 61 ggctgataaa agtggagccg agtctaatac ccactcttga gctgccccag gatcaagatt 121 gccatgaaat gtggtccaag cgtcgtcgtc aagcacagcg gcaaaacgcc gggatgctgg 181 agaaacgata acagtgactt tagagcttca ggttgcagcg gcatagcgtg tttttattgg 241 tatacatttg cttcacgtgc gcatatgttt tgaattaaac attgaacgaa ca //