LOCUS       EY194028                 479 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab01h04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194028
VERSION     EY194028.1  GI:159496600
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 666
            PolyA tail found inside read...SR being truncated to 630
            Potential full insert read...vec-to-vec length: 551
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggaa agttctgctc aaagagcctc aattccacag cgtgatcgat tgcttccaag
       61 gttggccgaa gcatcatgtc ccaaacgatg tctggtggta ctacataatc gagtcgggat
      121 tctattgggc tcttctcttc agcactcatg atggttctgt gcctactctt gatcctccac
      181 ctcttctgga cctatttgct cgtcaagatc gctatcaaat cactgaaaag tggcgttgat
      241 gatatccgag aggaaagtga gggtgaagag tatgaagaag agcggacaaa gaaagataag
      301 tgatctgtgc cttaggcatt tttggcagca gcaattcatc ctttattatc tcaatgcctt
      361 ttgttgattg gtagtttctt tcaattctag agatttttat cgcctcaaca gaaacaggaa
      421 aataattata tcttgccctt gtccattcaa gagtaataat tgcgataact gataaatca
//