LOCUS       EY194027                 435 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab01g11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_502655.1| Ribosomal Protein, Large subunit RPL-18
            (21.0 kD) (rpl-18) [Caenorhabditis elegans] pir|T26939 hypothetical
            protein Y45F10D.12 - Caenorhabditis elegans emb|CAA16387.1| C.
            elegans RPL-18 protein (corresponding sequence Y45F10D.12), mRNA
            sequence.
ACCESSION   EY194027
VERSION     EY194027.1  GI:159496571
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 791 was extended to value 792 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 147
            original value before phred recall for SR was 626
            PolyA tail found inside read...SR being truncated to 586
            Potential full insert read...vec-to-vec length: 507
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggtt tgtgtgggcg ctatcaccga cgacaagcgc atctacaaag tcccggccat
       61 gactatctgc gctctgcgct tcaccagctc tgcgcgtgct cgcatcatcg ctgcgggtgg
      121 agagaccatc acattggacc aattggcgct gcgcgcaccg aagggcgaga acacgctgct
      181 cttgcagggc aagcgcaagg cacgcgaagc ggagaagcat ttcggaccgc cgcctggtgt
      241 acatggcagc catgccaagc cattggttcg cgccaaagga agaaagttcg aacgcgctcg
      301 tggtcgccga aagtcgcgtg gctacaagaa ctgatgacga agtcatttgc ggttggagag
      361 gagcaaacat cttcgagagg agacaacgga ctttacattg ttgttattgt taacaaacgg
      421 ataaaaattg atgca
//