LOCUS       EY194019                 424 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab01f11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194019
VERSION     EY194019.1  GI:159496563
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 819 was extended to value 819 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 620
            PolyA tail found inside read...SR being truncated to 575
            Potential full insert read...vec-to-vec length: 496
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggac gctaaaacgt tgcaatggca tggaccaatg cacgaatggg aaggtgaaga
       61 aagcgataaa gtgcgggcag agatcagcgc agatggcgga gttatggcag tgcaaatagg
      121 atttaaaacg gagatgatcc gctgtttccg tctccactcc cattttgctg gaaaagtgcc
      181 ttctctggtc aattgcgctc ttttgtcact gcaatgttgt acagcaaacg ttgattgccg
      241 catcgaatgt tgtcaaatgc tcgcgcagct ggggttagat ttaagcaaat aatagaaact
      301 gctgaggagg aaagaaaaaa gatatcacag atcattcatt tttctttaaa atatggctgt
      361 ttgttgtatg ttagtggcat tgtgttttat tttgttaaac aacaaataaa tactgtatag
      421 tgca
//