LOCUS EY194018 388 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab01f09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to gb|AAP22042.1| glutamate carboxypeptidase-like protein 1 [Oreochromis mossambicus], mRNA sequence. ACCESSION EY194018 VERSION EY194018.1 GI:159496562 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 388) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 808 was extended to value 809 (,) putative full insert read: vector-to-vector length is 582 (,) This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 732 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..388 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggaa ggtgaccggt aaattcatca tcagaattgt gccggacatg gtgcccgacg 61 aggtagacaa ggtggtggtg caacacgtgc aagaggtgtg gaagcagcgg ggatcgccca 121 acaaaatgag cattctgccc cagggaggca ctctggcctg gctcggcgac ttcaaacatc 181 cgcacttcca ggcgggcgcg aatgcggtca aaaaggtgta cggagtggag ccggacttca 241 cccgtgaagg tggcagcatt cccgtcactc tcactttcca ggagatgacc ggaaagagtg 301 tgatgttgct gccgatgggc gcatcggatg acatggcgca ctcgcagaac gagaagttga 361 acgtgaccaa ctacatgcag ggaatgaa //