LOCUS       EY194016                 298 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab01f07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194016
VERSION     EY194016.1  GI:159496532
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 824 was extended to value 825 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 491
            PolyA tail found inside read...SR being truncated to 448
            Potential full insert read...vec-to-vec length: 370
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggtt cgaggcacgt gacctctact ccgtgtgcat gaccctgttg tcgttgggcc
       61 gaattatgga gaagaaggga aagccgaacc cattctctgg atgaagagta gaagtgggtg
      121 cagcaaaaga cggacagagc atgtgctatc gctcccattc gagaactccc cgttttgcga
      181 attttctccc cgtgtgcgac cttgcaacaa taccgaggcg ttaactgttt tcccctctct
      241 ctcctctcaa actgtgggca tttgaaaata gcgatgccgg aataaatggc caattcga
//