LOCUS EY194015 415 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab01f06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_498231.1| Ribosomal Protein, Large subunit RPL-23 (15.0 kD) (rpl-23) [Caenorhabditis elegans] sp|P48158|RL23_CAEEL 60S ribosomal protein L23 pir|T15337 hypothetical protein B0336.10 - Caenorhabditis elegans gb|AAK18857.1| Ribosomal protein, mRNA sequence. ACCESSION EY194015 VERSION EY194015.1 GI:159496531 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 415) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 845 was extended to value 846 (,) This trace has been recalled with phred original value before phred recall for SL was 147 original value before phred recall for SR was 630 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..415 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcgg gagcgaaatt ccgtgtcact ttgggtctcc cagtcggggc tgtaatgaat 61 tgcgccgata ataccggagc aaagaatctg tttgtcattg ccgtgtacgg gattcgcggt 121 cggcttaacc gacttccaag tgccgccgtc ggagacatgt ttgtgtgttc cgtcaaaaaa 181 ggcaaaccag agctgcgcaa aaaagttctc caagccgtag ttgtccgcca gcgaaaacaa 241 tatcgtcgga aggatggcac tttcatttac ttcgaggaca acgccggagt tatcgtcaac 301 aacaaaggcg agatgaaggg ctcggcgatt accggcccag tagccatgga gtgtgccgac 361 ctttggccga agatcgcctc taatgcgagt tctattgctt gaacggttgc gcata //