LOCUS EY194005 517 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab01e05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY194005 VERSION EY194005.1 GI:159498339 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 517) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 817 was extended to value 818 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 740 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..517 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcag ctagaggtgg tgttcgtgtc gtgggatcgc acttccgagg agatggatga 61 gtacttcaag gaagtgcacg gccagtggac gcacctcgag ttcgacaagg agtacttcga 121 gaaactgacg gagcggttcg acgtggatgg tattccttgt ctgaagcttg tccgtccgga 181 cggggccgtc accattgagg actgcagcga cgacctgcga ccggtagttt ggggattgct 241 aaatgtccca ctagttttgg tccagccacg cggcggagac gacggccgca cctgcgatgt 301 gtgccacaac gaggtggaga ccacctggtg gcagtgcacg gtctgcaggc cggcgtacga 361 cctgtgcctc aagtgcaaaa cgcactttgt gctgagccag gagcacaagt tcgaggaaaa 421 gcaaaagagg cagccggaga agaagactgc cgcccagatt tgggacaagt ggatggatga 481 gtaccagaaa gagaattgat tgattgatgg gtgagcg //