LOCUS       EY193866                 342 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa99e08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_505800.1| Glutathione S-transferase, C-terminal
            domain and Elongation factor 1 gamma, conserved domain containing
            protein (44.4 kD) [Caenorhabditis elegans] sp|P54412|EF1G_CAEEL
            Probable elongation factor 1-gamma (EF-1-gamma) (eEF-1B gamma),
            mRNA sequence.
ACCESSION   EY193866
VERSION     EY193866.1  GI:159498374
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 537
            PolyA tail found inside read...SR being truncated to 493
            Potential full insert read...vec-to-vec length: 414
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggca ccaagtggcg gaaagaaaga aaagaagcag gagaagcagc aaaaagagcc
       61 caagccgcaa gcggaaaaac aaccaaagaa gaaactcgac ccgaatgaag aagcgacgaa
      121 gaacatggtc aaagagtact ttatgtggga gggcgaattt ggcggaaaga aattcaacca
      181 gggcaagatt ttcaagtagt gacggaactt gattactaat tgacacgctt acaaatttca
      241 aaaaagtgga tgcgtgtgca ttaagtgtta catgttgctt gttattttat cttttggtta
      301 ttgttggata aagttggaaa aaaaaaaaaa aaaaataaaa ta
//