LOCUS EY193864 529 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa99e06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_499799.1| Fatty acid ligase Long (75.8 kD) [Caenorhabditis elegans] pir|T27421 hypothetical protein Y76A2B.3 - Caenorhabditis elegans emb|CAA21744.1| Hypothetical protein Y76A2B.3 [Caenorhabditis elegans], mRNA sequence. ACCESSION EY193864 VERSION EY193864.1 GI:159498372 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 529) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 825 was extended to value 825 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 831 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..529 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggaa gcgctggctg ttctcgctgg cgcacgcgca gaagttggcg ctgctgcgca 61 agggcgtcgt caccaaccag acggtgtggg acaaactggt gtttgtgaag gcgcaggagc 121 agctgggcgg caaggtgcgc ctcatcatca ccgggtcggc gccgatcgcg gccgaagtgc 181 tgcaggactt gcgcatcgcg ttcggcacca ccatcgtcga gggctacggc cagaccgagt 241 gcacagccat ggtgacgtgc acattgcccg gcgacgtgga gggcggccac tgtgggccgc 301 cggccgtgtg ctcgctgctc aagctggccg acgtgcccga cctgaactat tttgcgaaag 361 atcgcaaggg cgaagtgatg gtgaagggcc cgatggtgac caagggctac tacagggacg 421 cggagaagac ggcggagctg ttcgacgctg atggcttcct gcacaccggg gacattggcc 481 acattttggc ggacggccgc ctcaagaaca tcgaccgcaa aaagcacat //