LOCUS       EY193862                 461 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa99e02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193862
VERSION     EY193862.1  GI:159498359
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 818 was extended to value 819 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 690
            PolyA tail found inside read...SR being truncated to 612
            Potential full insert read...vec-to-vec length: 533
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggacc gatgacagcg cagaaagagg tagccagcac cgccagctcg tccacacgag
       61 caaattctac cagcagggac agctggagtt gttcatcgac aaactgtcac agcgcttctt
      121 ggctgccacc aagccgagtg cttttgcgtt ttcgattcaa catctcggag catctaatcg
      181 agttcgagcg gttcaacacg tgcaccggag aagtgctgct gctgaaaatg caggtggaga
      241 agaaaaagaa gaaagccggc agctatttgc tccaaagacg accaaacgga gaacaacaag
      301 gggaggaagc caaccattgg attgaatgcg cgatgaagaa tgacaaccca atttcgttat
      361 atctttgaag gtggcccgat tctgtaattt aacttttaac agaccgttcg ttaactttgt
      421 tggtgcccaa gaggtgcaaa taaataaaac tcttttaaat a
//