LOCUS EY193859 476 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa99d10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_524793.2| myospheroid CG1560-PA [Drosophila melanogaster] sp|P11584|ITBX_DROME Integrin beta-PS precursor (Position-specific antigen, beta chain) (Myospheroid protein) (Olfactory C protein) gb|AAM29504.1| RE55238p [Drosophila melanogaster], mRNA sequence. ACCESSION EY193859 VERSION EY193859.1 GI:159498356 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 476) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 644 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..476 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggtac gacggggcgg acaacctgac cgtctgggtc aaggagcaca aagattgtcc 61 ggcgccgttg cccgtgctgg tcattgtgct catcgtgatc gcgggcattg tgctgctggg 121 gctgctgctg ctgctgctgt ggaagctgct caccgtcatc tacgaccggc gcgagttcgc 181 gcgcttcgag aaggaccgac tgaatgcgag atgggacacg aacgagaacc cgctgtacaa 241 gcaggcgacc accacattca agaacccagt gtactcggga gacgcggcca agaacaagtg 301 acgggcggga ttagacggag cagagaatgt tagctgagaa caaaaccaag tgcccctcct 361 ttgaaggaaa cagccatttc gcggaaagac gagactggtc aaatcgcgga aatcaaacaa 421 agctcattca aagctgagta tttccttttt ttgaccagct ctttttcccc ttcctc //