LOCUS EY193858 260 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa99d09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_740927.1| PMR-type Golgi ATPase PMR-1 (pmr-1) [Caenorhabditis elegans] emb|CAC19895.1| PMR1 protein [Caenorhabditis elegans] emb|CAC19896.1| PMR1 protein [Caenorhabditis elegans] emb|CAB04015.2| C. elegans PMR-1 protein (corresponding seque, mRNA sequence. ACCESSION EY193858 VERSION EY193858.1 GI:159498355 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 260) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 152 original value before phred recall for SR was 443 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..260 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggtc cggtgctcaa atcgatgaaa tgacagactt ggagcttgag cgcatcatcc 61 gagctgtttc cattttctac cgcgcctcgc caagacacaa attgcgcata gtgaaggcac 121 ttcagaacat tggcgaggtg gtcgcgatga ccggcgatgg agtgaatgat gcggttgcac 181 tgaagaagtc ggacattggc gttgcaatgg gcgaatcgag aacaggcgtg tgcaaagagg 241 cagcggacat ggtgctcatc //