LOCUS EY193856 572 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa99d07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193856 VERSION EY193856.1 GI:159498353 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 572) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 787 was extended to value 788 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 792 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..572 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggcc aaaggaacag caggagcaaa cagcacagca accaatggtt cagcagcaac 61 ccttccaaaa atggttcccg gaagtgcctt ctgtgttttt ccaggtcgat gatccaaacg 121 gcaacagcga cgaggcgagc aacagtaaca agccggatag tgcccaacct gagcaacaac 181 gtccacaaca gctgccgcaa caacagcagc aacaaagcgc gccacagatg actgaatgaa 241 tgtgggaaat tgaatcggga aatgaaatga gtgaatttgt tttgagttaa agaacctgaa 301 accccataca tattcataac atgcccatcc tcactagttt ccatttcatt atcaattatc 361 attgccccat aaaacaaaaa agtgcgtgct ccgaaattga tcaaaacgac ataaaacaat 421 cgccttcgat gtgctacaac cgttgtgatg atgaatcgaa ttgcagagct gcattcttaa 481 attgcattaa agtaccacaa tcgataaccg cagttgattt attcattcct aaccctgatt 541 aagtatattt ctcgattttg tttccgactc at //