LOCUS EY193855 574 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa99d06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193855 VERSION EY193855.1 GI:159498352 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 574) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 841 was extended to value 842 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 847 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..574 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggaca gtgcacagtg tgtacgaccg acgcaaccga gtgcacgtca tccacgtcag 61 cacgagagac cctacgaacc ggtgaatgtg ttcggcagtg agacgctgcg caaaataatg 121 gcagaagagc gcgtcaaact caaagaggag acggacggct ctgtcgcgga agaggacgaa 181 ttgggggaca gcgactacgg agaagagaca gaatgggaca gcgactcgtc cgcagaggac 241 gcctcttttg tggaggagga ccaacaggtg tcggcgcaga tctcgcgcaa acgcgacaac 301 gaaaaacaaa tggccaaggc caacaaagtg gccaaaaatc gcatggaaca gcgccagcgc 361 tcttcatcgg tttcgaaaga gcgaagcgaa gaactggagg aggtgcttgg cgacgagtac 421 tttgacagcg cctactatgc gccggcgtcc gcttcacttg tgcactggtt gtccctggaa 481 tggctcatct tgatggcatt cgctttgatg ggcacgccgt ttttggccat ctaaactcca 541 tttgaaaaac acaattattt tttgcgcatt cgat //