LOCUS EY193770 460 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa98c10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_075638.2| cytosolic nonspecific dipeptidase [Mus musculus] sp|Q9D1A2|CGL1_MOUSE Cytosolic nonspecific dipeptidase (Glutamate carboxypeptidase-like protein 1) dbj|BAB22991.1| unnamed protein product [Mus musculus] gb|AAH05532.1| RIKEN cDNA 0, mRNA sequence. ACCESSION EY193770 VERSION EY193770.1 GI:159496064 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 460) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 147 original value before phred recall for SR was 652 PolyA tail found inside read...SR being truncated to 611 Potential full insert read...vec-to-vec length: 532 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..460 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcaa gaggtgtgga agcagcgggg atcgcccaac aaaatgagca ttctgcccca 61 gggaggcact ctggcctggc tcggcgactt caaacattcg cacttccagg cgggcgcgaa 121 tgcggtcaaa aaggtgtacg gagtggagcc ggacttcacc cgtgaaggtg gcagcattcc 181 cgtcactctc actttccagg agatgaccgg aaagagtgtg atgttgctgc cgatgggcgc 241 atcggatgac atggcgcact cgcagaacga gaagttgaac gtgaccaact acatgcaggg 301 aatgaagatg ttcgctgcct acctgttcga gtgcgccaaa atctgaatgg acgtagacgg 361 gatgaggaag ggaaaccaaa cagtgttgtt accatgatat ccacacgtaa taactaataa 421 ccatggacaa cttgagcaaa ataaagggaa ttgttctgga //