LOCUS       EY193754                 462 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa98b03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193754
VERSION     EY193754.1  GI:159496048
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 841 was extended to value 842 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 654
            PolyA tail found inside read...SR being truncated to 613
            Potential full insert read...vec-to-vec length: 534
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggac aaccgaacaa caaaacaaca tccactctct ccctccctct tttgaccaaa
       61 aactgtattt cccaacccaa aaaatggacc aatcggccat tctgaaattc atcgccggtc
      121 ggctgtcgca gcccctgacc gaggcagaga gcgaactttt gcacgccctg atccgaaatt
      181 ccatcggtcc aaagcaaatc catccgaatt tgccgaaacg acgccgctcc cagattgcct
      241 tctatttctc ctccctgcac cacattgtgg aggagccaga ggaagaagtg ccggaggggc
      301 acttggaacg ggcgaggagc tgttcgcgga caatgtccat tggcagcgcc aaacagttcc
      361 gagggaagga gccacaaatg caaatgatca gaaatgtctg aaaacccaat tatttgattc
      421 gcaaaatata aacggcaaaa aatgagcgaa aatatttagc ca
//