LOCUS EY193739 174 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa97h10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193739 VERSION EY193739.1 GI:159496033 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 174) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 817 was extended to value 817 (,) This trace has been recalled with phred original value before phred recall for SL was 151 original value before phred recall for SR was 369 PolyA tail found inside read...SR being truncated to 327 Potential full insert read...vec-to-vec length: 246 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..174 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcgg acagagcatg tgctatcgct cccattcgag aactccccgt tttgcgaatt 61 ttctccccgt gtgcgacctt gcaacaatac cgaggcgtta actgttttcc cctctctctc 121 ctctcaaact gtgggcattt gaaaatagcg atgccggaat aaatggccaa tgca //