LOCUS       EY193724                 619 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa97g06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_491792.1| Phenylalanyl (F) tRNA Synthetase FRS-1
            (62.7 kD) (frs-1) [Caenorhabditis elegans] pir|T28752
            phenylalanine-tRNA ligase (EC 6.1.1.20) beta chain T08B2.9
            [similarity] - Caenorhabditis elegans gb|AAG00015.1| Phenylalanyl
            (f) trna syn, mRNA sequence.
ACCESSION   EY193724
VERSION     EY193724.1  GI:159496018
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 841 was extended to value 842 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 844
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggca cgacaccttc ttccttacaa ggccggctat ctccactgaa tttccgatgg
       61 actacctgga gcgggttaag aaggtccatt cggagggtgg atacgggtcc attggctacc
      121 aatatgattg gaaaattgaa gaggcccaga aaaacgtgtt gagaacgcac acaactgcag
      181 tttcggccaa acagctctat ttgttggctc aaaatggatt caagccgtcc aagatgttca
      241 gcatcgatcg ggtcttccgg aatgaaacgc tggacgccac tcatttggcc gagttccacc
      301 aggtggaagg cgttgtcgcg gagcggaatc tctcacttgc ccacgtgatc ggacttttca
      361 ccgagttctt ccgaaaatgc ggcattgaaa atctccgctt caagcccacc tacaacccgt
      421 acacagagcc ctcgatggag atattcgcct tccactacgg gctgaacaag tgggtggaga
      481 ttggcaattc gggcatgttc aggccggaaa tgctgctgcc aatgggactt cctgaggatg
      541 tggctgtggc gggattcggg ctgtcgctgg agaaccgacc atgatccggt acggcatttc
      601 gaacattcgg gagctgttc
//