LOCUS       EY193723                 425 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa97g05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193723
VERSION     EY193723.1  GI:159496017
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 843 was extended to value 844 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 618
            PolyA tail found inside read...SR being truncated to 575
            Potential full insert read...vec-to-vec length: 497
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggagg tgcaacaatt ccttcggttt tgccacccat accttcgaat ggcaaacgaa
       61 gcgaaaaagt tgaacagcaa gaccgaatac ttggcaaaaa gtcttatgtc aaatgtctgg
      121 gaggtcttaa taaaggcgcg tacggacaga ttacaagcat ggatgaagaa aattcgagtg
      181 cttttgtgga gctgagttgg ccaccggaaa atgcagggaa gatagtgcgc gtaagccagt
      241 tcactttgga gattgtcgga cagagggagt tccgcgacaa aactgaacgt atggaagaca
      301 agaagaaatg gtaacaaaac ggccagagtt tgatgttccg aatgtgtggt tgaacgagaa
      361 ctcaagtcaa tcccaaatcc taatgtcacc tatgggattt aaaaggataa agttggattg
      421 atata
//